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KMID : 0620920070390050641
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Experimental & Molecular Medicine
2007 Volume.39 No. 5 p.641 ~ p.652
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Identification of molecular markers for the oncogenic differentiation of hepatocellular carcinoma
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Yu Gyung-Ran
Kim Seong-Hun
Park Seon-Hwa
Cui Xiang-Dan
Xu Dong-Yuan
Yu Hee-Chul
Cho Baik-Hwan
Yeom Young-Il
Kim Sang-Soo
Kim Sang-Bae
Chu In-Sun
Kim Dae-Ghon
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Abstract
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The aim of this study was to identify molecular markers associated with oncogenic differentiation in hepatocellular carcinoma (HCC). Using an unsupervised clustering method with a cDNA microarray, HCC (T) gene expression profiles and corresponding non-tumor tissues (NT) from 40 patients were analyzed. Of total 217 genes, 72 were expressed preferentially in HCC tissues. Among 186 differentially regulated genes, there were molecular chaperone and tumor suppressor gene clusters in the Edmondson grades I and II (GI/II) subclass compared with the liver cirrhosis (LC) subclass. The Edmondson grades III and IV (GIII/IV) subclass with a poor survival (P = 0.0133) contained 122 differentially regulated genes with a cluster containing various metastasis- and invasion-related genes compared with the GI/II subclass. Immunohistochemical analysis revealed that ANXA2, one of the 72 genes preferentially expressed in HCC, was over-expressed in the sinusoidal endothelium and in malignant hepatocytes in HCC. The genes identified in the HCC subclasses will be useful molecular markers for the genesis and progression of HCC. In addition, ANXA2 might be a novel marker for tumor angiogenesis in HCC.
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KEYWORD
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angiogenesis, carcinoma, hepatocellular, cell differentiation, gene expression profiling, tumor markers, biological
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